Titlebook: HIV Reservoirs; Methods and Protocol Guido Poli,Elisa Vicenzi,Fabio Romerio Book 2022 Springer Science+Business Media, LLC, part of Springe

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Methods in Molecular Biologyhttp://image.papertrans.cn/h/image/420200.jpg

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Comparative Government and Politics1 infection at the mucosal level. Beyond pregnancy, the decidua constitutes also a valuable tool to assess tissue-resident macrophage infection. Here, we provide a detailed protocol for decidual macrophage purification and tissue infection.

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Hematopoietic Stem and Progenitor Cells (HSPCs)ible latently infected cells for reactivation studies. Infected HSPCs can also be found in the setting of clinically undetectable viremia in vivo. Here we describe an in vitro infection model utilizing cord blood derived HSPCs, as well as methods for isolating and characterizing provirus from bone marrow HSPCs from suppressed patients.

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Decidua Basalis: An Ex Vivo Model to Study HIV-1 Infection During Pregnancy and Beyond1 infection at the mucosal level. Beyond pregnancy, the decidua constitutes also a valuable tool to assess tissue-resident macrophage infection. Here, we provide a detailed protocol for decidual macrophage purification and tissue infection.

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https://doi.org/10.1057/9780230582217ant in the late 1980s and early 1990s when most experimental in vitro infections were based on laboratory-adapted “T-cell tropic” strains of HIV-1, such as LAI/IIIB or others, that later were found to rely upon CXCR4 as coreceptor for viral entry in addition to CD4 as primary receptor. Although prim

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https://doi.org/10.1007/978-3-642-37238-4 immune responses and to persist during antiretroviral therapy, which represents an important problem in clinical practice. Here we describe both the original and a simplified version of HIV-1 latency models that mimics this process using replication competent viruses. Our model allows generation of