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Titlebook: HIV Reservoirs; Methods and Protocol Guido Poli,Elisa Vicenzi,Fabio Romerio Book 2022 Springer Science+Business Media, LLC, part of Springe

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书目名称HIV Reservoirs
副标题Methods and Protocol
编辑Guido Poli,Elisa Vicenzi,Fabio Romerio
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: HIV Reservoirs; Methods and Protocol Guido Poli,Elisa Vicenzi,Fabio Romerio Book 2022 Springer Science+Business Media, LLC, part of Springe
描述.This book details the development of methods and models to study the HIV-1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts covering cell lines, .in vitro. and .ex vivo .primary cell models of persistent infection, .in vitro. and .ex vivo .tissue-derived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cART-free long-term remission. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols... ..Authoritative and cutting-edge, .HIV Reservoirs: Methods and Protocols. provides a comprehensive, updated collection of state-of-art methodologies and models to tackle the HIV-1 viral reservoir..
出版日期Book 2022
关键词Primary non-CD4+ T lymphocyte; cervical tissue; animal models; QVOAS; TILDA
版次1
doihttps://doi.org/10.1007/978-1-0716-1871-4
isbn_softcover978-1-0716-1873-8
isbn_ebook978-1-0716-1871-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2022
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https://doi.org/10.1057/9780230348660lood naïve CD4T cells from peripheral blood with a phenotype resembling that of central memory CD4T cells. This model can be used to investigate the mechanisms involved in latency as well to develop strategies to target it.
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The Cultured TCM Model of HIV Latencylood naïve CD4T cells from peripheral blood with a phenotype resembling that of central memory CD4T cells. This model can be used to investigate the mechanisms involved in latency as well to develop strategies to target it.
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Human Monocyte-Derived Macrophages (MDM): Model 1 (GM-CSF)ablishment of the “hard-core” reservoir, and in their capacity to cause a low-level virus production during cART. Here, a protocol is presented for cell culture and HIV-1 infection of granulocyte–macrophage colony-stimulating factor (GM-CSF) differentiated human monocyte-derived macrophages.
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https://doi.org/10.1057/9780230582217ould be used to investigate features of proviral reactivation, as reviewed in (Cassol et al. J Leukoc Biol 80:1018–1030, 2006), two cell lines in particular have been broadly used and will be here discussed: the U937-derived U1 and HL-60–derived OM-10.1.
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https://doi.org/10.1057/9780230801400y and reversion assay (LARA) culture conditions uniquely retain phenotypically and transcriptionally distinct memory CD4+ T cell subsets that allow in a single assay to assess LRA activity in each memory subset and differential examination of the dynamics of HIV latency reversal.
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