Titlebook: HIV Protocols; Vinayaka R. Prasad,Ganjam V. Kalpana Book 2016Latest edition Springer Science+Business Media New York 2016 HIV-1 RNA struct

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Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seqify the RNA molecules that are bound by the HIV-1 Gag protein in cells and in virions. This protocol can easily be applied to other viral and cellular RNA-binding proteins that influence HIV-1 replication.

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Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integrasees for the inhibitor-induced aberrant IN multimerization, a dynamic light scattering-based assay which allows for monitoring the formation and sizes of oligomeric IN particles in a time-dependent manner, and a chemical cross-linking-based assay of interacting IN subunits which allows for the determi

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High-Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and Pathogenesisase (HIV-LucR) and enables investigators to use bioluminescence to visualize and quantitate the temporal effects of therapeutics on HIV-1 infection. The second model, hCD4/R5/cT1 mice, consists of transgenic mice carrying human CD4, CCR5 and cyclin T1 genes, which enables murine CD4-expressing cells

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https://doi.org/10.1007/978-3-642-11522-6uorescent virus particles, can be used to measure cell-to-cell transfer of virus particles. HIV NL-GI, a clone that expresses GFP as an early gene, facilitates the measure of productive infection after cell-to-cell contact. Lastly, a variation of the β-lactamase (BlaM)-Vpr fusion assay can be used t

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The Global Old Age Care Industryase (HIV-LucR) and enables investigators to use bioluminescence to visualize and quantitate the temporal effects of therapeutics on HIV-1 infection. The second model, hCD4/R5/cT1 mice, consists of transgenic mice carrying human CD4, CCR5 and cyclin T1 genes, which enables murine CD4-expressing cells

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https://doi.org/10.1007/978-3-319-66215-2CA-NC complexes (Fricke et al., J Virol 87:10587–10597, 2013). Here we describe in detail the use of this capsid stability assay. We believe that our assay can be a powerful tool to assess HIV-1 capsid stability in vitro.