Titlebook: ;

Malnutrition

书目名称Golden Gate Cloning影响因子(影响力)




书目名称Golden Gate Cloning影响因子(影响力)学科排名




书目名称Golden Gate Cloning网络公开度




书目名称Golden Gate Cloning网络公开度学科排名




书目名称Golden Gate Cloning被引频次




书目名称Golden Gate Cloning被引频次学科排名




书目名称Golden Gate Cloning年度引用




书目名称Golden Gate Cloning年度引用学科排名




书目名称Golden Gate Cloning读者反馈




书目名称Golden Gate Cloning读者反馈学科排名

有发明天才

Use of a Golden Gate Plasmid Set Enabling Scarless MoClo-Compatible Transcription Unit Assembly,erate transcription units. Typically, type IIS enzymes are used, which generate four nucleotide overhangs. This results in small scar sequences in hierarchical DNA assemblies, which can affect the functionality of transcription units. However, there are enzymes that generate three nucleotide overhan

半球

inveigh

Validation of Golden Gate Assemblies Using Highly Multiplexed Nanopore Amplicon Sequencing,libraries, resulting in assemblies of high combinatorial complexity where sequencing again becomes mandatory. Here, we present a detailed protocol for the use of a highly multiplexed dual barcode amplicon sequencing using the Nanopore sequencing platform for in-house sequence validation. The workflo

Ptosis

Modular Golden Gate Assembly of Linear DNA Templates for Cell-Free Prototyping,tion of a repressor–promoter pair. By eliminating time-consuming transformation and cloning steps in cells and by taking advantage of modular cloning toolboxes, our cell-free prototyping workflow can produce data for large numbers of new assembled constructs within a single day.

convert

convert

BIDE

Lumbar-Spine

Rosemarie Klemm,Dietrich D. Klemming the type of part that needs to be cloned, (2) designing primers for amplification, (3) performing polymerase chain reaction (PCR) amplification, (4) cloning of the fragments using Golden Gate cloning, and finally (5) sequencing of the part. For large standard parts, it is preferable to first clo

coagulation