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发表于 2025-3-21 16:27:02 | 显示全部楼层 |阅读模式
书目名称Group A Streptococcus
编辑Thomas Proft,Jacelyn M. S. Loh
视频video
丛书名称Methods in Molecular Biology
图书封面Titlebook: ;
出版日期Book 2020
版次1
doihttps://doi.org/10.1007/978-1-0716-0467-0
isbn_softcover978-1-0716-0469-4
isbn_ebook978-1-0716-0467-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
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发表于 2025-3-21 21:01:11 | 显示全部楼层
https://doi.org/10.1007/978-3-030-39407-3presented approach can be used for the analysis of all subcellular proteomes from . and enables identification of drug or vaccine targets within a certain cellular fraction. Here, proteins integral to the plasma membrane are of particular interest for the development of new antimicrobial therapies.
发表于 2025-3-22 01:47:42 | 显示全部楼层
Protocol for Proteome Analysis of Group A Streptococcuspresented approach can be used for the analysis of all subcellular proteomes from . and enables identification of drug or vaccine targets within a certain cellular fraction. Here, proteins integral to the plasma membrane are of particular interest for the development of new antimicrobial therapies.
发表于 2025-3-22 04:44:22 | 显示全部楼层
https://doi.org/10.1057/9780230276086iplex PCR reactions to detect genes encoding 20 virulence factors: .3, ., .B, .D, .B, .CEP, .A, ., sic, .L, .K, .M, .C, .I, .A, .H, .G, .J, .Z, and .. Classification of strains based on the virulence factors absence or presence correlates with PFGE MLST and emm typing results. The typing/detection s
发表于 2025-3-22 08:58:16 | 显示全部楼层
发表于 2025-3-22 15:52:27 | 显示全部楼层
Hysterical Hypnosis and Infectious Theatre,siology. The Tn-seq approach allows the . monitoring of highly complex mutant libraries, leveraging massive parallel DNA sequencing as a means to characterize the composition of these mutant pools on a genome-scale with unprecedented nucleotide-level high resolution. In this chapter, we present step
发表于 2025-3-22 20:02:38 | 显示全部楼层
Performing Objects and Theatrical Thingsdifferent strains. Here, we outline an optimized, rapid method for creating markerless isogenic mutations that combines Gibson assembly cloning with a new temperature-sensitive plasmid, pLZts. This method is highly efficient and reduces the time needed to create GAS mutants to ~2–3 weeks, with the a
发表于 2025-3-22 22:14:59 | 显示全部楼层
https://doi.org/10.1007/978-3-319-96701-1ethod for labeling GAS with a set of plasmids we have developed and deposited with Addgene. These plasmids can be used to either integrate firefly luciferase (Ffluc) or red-shifted firefly luciferase (FflucRT) into the GAS genome or to introduce the reporters on plasmids which have been stabilized w
发表于 2025-3-23 05:18:10 | 显示全部楼层
https://doi.org/10.1007/978-981-10-7998-6nical advancements and the development of new bioinformatic tools for analyzing genomic data; however, the basic principles and processes for defining and processing high-quality genome sequence information remain unchanged. Here, we introduce some considerations and describe some commonly used bioi
发表于 2025-3-23 06:47:56 | 显示全部楼层
Michael Lambert,Tamantha Hammerschlagies, real-time PCR or microarray studies were the mainstay of assessing differences in gene expression in bacteria. Real-time PCR remains a critical tool for targeted gene expression analyses. However, microarray studies have given way to the plethora of advantages in RNA sequencing (RNA-seq) for th
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