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楼主: FLAK
发表于 2025-3-23 09:49:52 | 显示全部楼层
Circular Permutation of the Green Fluorescent Proteinng in intact cells and organisms (.–.). GFP represents the first genetically encoded reporter molecule that is detectable in the absence of an enzymatic substrate or cofactor, in a variety of cell types.
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Fluorescence Lifetime Imaging (FLlM) of Green Fluorescent Fusion Proteins in Living Cellstics, sensitive fluorescent dyes, and high-sensitivity cameras, coupled with technological advances in computers and sophisticated software now permit quantitative measurement and noninvasive acquisition of spectroscopic information from a single living cell (.–.). Because of the specificity inheren
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Light-Induced Nuclear Targeting of PhytochromeB-sGreen Fluorescent Protein in Plants20 kDa, to which a linear tetrapyrrole chromophore is covalently attached. The holoprotein undergoes photoreversible conversion between two spectrally distinct forms, the far-red light-absorbing form (Pfr) and the red light-absorbing form (Pr). Of these two forms, Pfr is biologically active. Red lig
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