Titlebook: Golgi; Methods and Protocol Yanzhuang Wang,Vladimir V Lupashin,Todd R. Graham Book 2023 The Editor(s) (if applicable) and The Author(s), un

Blazon

glamor

Studying Golgi Structure and Function by Thin Section TEMM) and for 3,3′-diaminobenzidine (DAB) cytochemical staining and pre-embedding immunolabelling to localize specific Golgi proteins. Furthermore, we demonstrate how the Golgi morphology can be elucidated by classifying the Golgi membranes using Microscopy Image Browser—a software that provides anonymization, modelling, and annotation.

无思维能力

粗野

Reconstitution of Golgi Biogenesis in Permeabilized , CellsThe protozoan parasite, ., offers a simple system to study the growth and duplication of the Golgi. Cell lines stably expressing a photoactivatable GFP attached to an endogenous Golgi protein are permeabilized using digitonin. Photoactivation followed by imaging can then be used to follow the formation of the new Golgi.

nerve-sparing

archaeology

压舱物

Pageant

Quantification of Golgi Protein Mislocalization to the Budding Yeast Vacuoleuch larger vacuole is problematic for thresholding the images prior to calculating the MCC. In this chapter, we describe the use of Multi-Otsu thresholding in ImageJ to quantify the degree of GFP-tagged protein mislocalization to the vacuole. Furthermore, these methods can be applied to other colocalization events within the cell.

Redundant

Generation of GM130 Conditional Knockout Mouses established through gene targeting, stem cell screening, and blastocyst injection. Such model has been successfully applied for studies of physiological functions of GM130 and Golgi apparatus at the cellular and animal levels.

Resistance

Studying the Organization of the Golgi by Super-Resolution MicroscopyHere, we summarize our method to identify en face and side views of ministacks. It takes advantage of the characteristic ring and double-punctum staining patterns exhibited by cisternal rim-localized proteins. After averaging multiple en face views, the resulting image reveals the intrinsic organization of cisternae in a non-biased manner.