Titlebook: Glucose Transport; Methods and Protocol Karin Lindkvist-Petersson,Jesper S‘Hansen Book 2018 Springer Science+Business Media LLC 2018 GLUTs.

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Jeffrey A. Cardille,Monica G. Turnerology of biological cells after uptake of glucose. Here, we briefly discuss the advantages of using microfluidic systems. We further describe how microfluidic systems are fabricated and how they are utilized. Finally, we discuss how the large amount of data can be analyzed in a “semi-automatic” mann

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Jeffrey A. Cardille,Monica G. Turnerput screening. We generated a strain deficient for all endogenous hexose transporters, which has been successfully used to clone, characterize, and engineer carbohydrate transporters from different source organisms. Here we present basic protocols for handling this strain and characterizing sugar tr

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https://doi.org/10.1007/978-3-658-34495-5e last decade, the method has successfully been used to explore GLUT4 translocation in adipocytes. Here, we describe the procedure for studying GLUT4 trafficking using TIRF microscopy in isolated primary adipocytes.

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Ancient Assets for the Twenty-First Centuryas become an important tool in the assessment of GLUT4 recycling in cultured adipocytes and myocytes. Through the use of light microscopy, this reporter construct allows for visualization of GLUT4 specifically at the cell surface or GLUT4 that has recycled from the cell surface while simultaneously

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Leslie P. Steffe,Heide G. Wiegeln and muscle contraction to achieve a new steady state with more GLUT4 proteins at the muscle cell surface. To gain a better understanding of the molecular and cellular mechanisms that govern GLUT4 protein recycling, we developed an in vitro model in which .-epitope-tagged GLUT4 or GLUT4-GFP is expr