Titlebook: Glaucoma; Methods and Protocol Tatjana C. Jakobs Book 2018 Springer Science+Business Media LLC 2018 Intraocular pressure.Neuroprotection.Op

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Noninvasive Intraocular Pressure Measurement in Animals Models of Glaucoma,ls uses methods that have been adapted from use on humans. Calibration of the instruments used for the specific animal and even strain used is critically important for allowing meaningful comparisons of results. We describe below the methods used for noninvasive IOP measurement in animals that are relevant to glaucoma research.

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Quantitative Proteomic Analysis of Human Aqueous Humor Using iTRAQ 4plex Labeling,y (UHPLC-MS/MS) and data analysis for peptide identification and quantitation using Proteome Discoverer software. The protocol can be applied for other animals as well; however, pooling of specimens may be required to obtain sufficient amount of protein for labeling.

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Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma,ibe the use of anterograde neuronal tract tracing using cholera toxin B (CTB) for the determination of axon transport integrity of the retinofugal projection. This method reveals the structure of the retinal axons as well as the functional integrity of anterograde transport systems.

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https://doi.org/10.1007/978-1-4684-1587-2ms of optic nerve injury that occur in glaucoma. In addition, such a model could provide a valuable method for testing potential neuroprotective treatments. This paper outlines the basic methods for producing obstruction of aqueous humor outflow and IOP elevation by injecting hypertonic saline (a sc

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Chenxin Zhang,Liang Liu,Viktor Öwallation in glaucoma is associated with sensitivity to intraocular pressure (IOP) and elevated IOP (also known as ocular hypertension) is the primary modifiable risk factor. Ocular hypertension is the primary characteristic of rodent models for glaucoma research. Intracameral injection of microbeads ha

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Fei Richard Yu,Helen Tang,Hong Ji increasing the post-trabecular resistance to aqueous outflow by cauterizing the episcleral veins (EVC). This method allows the observation of ultrastructural changes in the trabecular meshwork (TM) without interfering with any structure within the eye such as TM, ciliary body, and/or the Retina. Th

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Heterogeneous and Liquid Phase Processes the disease is important for any research work trying to understand the pathophysiologic mechanisms of glaucoma. Noninvasive IOP measurement in animals uses methods that have been adapted from use on humans. Calibration of the instruments used for the specific animal and even strain used is critica

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