Titlebook: Germline Stem Cells; Michael Buszczak Book 2017Latest edition Springer Science+Business Media New York 2017 Germline stem cell.primordial

lactic

https://doi.org/10.1007/978-1-4842-8170-3technique for the acquisition of 4D datasets of the . ovariole for periods that can exceed 12 consecutive hours. Live-cell imaging facilitates the investigation of molecular and cellular dynamics that were not previously possible using still images.

APO

Script Component Transformation method to analyze poly(A) tail lengths of specific mRNAs in this tissue is valuable. Several methods of poly(A) test (PAT) assays have been reported to measure mRNA poly(A) tail lengths in vivo. Here, we describe two of these methods (PAT and ePAT) that we have adapted for . ovarian germ cells and germ-line stem cells.

placebo-effect

Per Andersson,Lars-Gunnar Mattssonmethod is presented here for identifying germ-line stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ hybridization to detect . mRNA and immunofluorescence to detect the pan-germ cell marker Vasa.

使高兴

Obstreperous

CBC471

Measurement of mRNA Poly(A) Tail Lengths in , Female Germ Cells and Germ-Line Stem Cells, method to analyze poly(A) tail lengths of specific mRNAs in this tissue is valuable. Several methods of poly(A) test (PAT) assays have been reported to measure mRNA poly(A) tail lengths in vivo. Here, we describe two of these methods (PAT and ePAT) that we have adapted for . ovarian germ cells and germ-line stem cells.

灾难

Identification of Germ-Line Stem Cells in Zebrafish,method is presented here for identifying germ-line stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ hybridization to detect . mRNA and immunofluorescence to detect the pan-germ cell marker Vasa.

CLASH

ZEST

Analysis of the , Germline Stem Cell Pool,the germline in living and fixed worms, cell cycle analysis, and analysis of markers. We also discuss assays to separate mutants that affect the stem cell vs. differentiation decision from those that affect germ cell processes more generally.

清晰

https://doi.org/10.1007/978-1-4471-0963-1 we describe the use of a monoclonal antibody to Pax7 as a means to detect spermatogonial stem cells (SSCs) both in tissue sections and in intact seminiferous tubules. Furthermore, we describe methods for lineage tracing as an alternative method to visualize Pax7. spermatogonial stem cells and their progeny.