Titlebook: Germline Stem Cells; Methods and Protocol Michael Buszczak Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under e

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Targeting Endogenous Loci That Function in , Germline Stem Cells,e other genetic manipulations is useful for studying germline stem cell biology. In this chapter, we will describe a method to use CRISPR-Cas9 genome editing technology to make knock-out and knock-in flies. We will cover everything from guideRNA (gRNA) and donor plasmid design and cloning to screening for positive edits.

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名义上

Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells, PSCs with distinct properties. By modulating the FGF, TGF-β, and WNT pathways, we have derived intermediate PSCs (FTW-PSCs) that are permissive for direct primordial germ cell-like cell (PGC-LC) induction in vitro. Here, we describe the method for derivation and maintenance of mouse and human FTW-PSCs, as well as PGC-LC induction from FTW-PSCs.

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