书目名称 | Germ Cell Protocols | 副标题 | Volume 1: Sperm and | 编辑 | Heide Schatten | 视频video | | 概述 | Includes supplementary material: | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | All sexually reproducing organisms produce primordial germ cells, a small population of cells that differentiate into gametes of either sex and carry to- potency, an ability to develop into an entire new organism. The study of germ cells has undergone enormous advances in recent years and has entered into an explosive phase of new discoveries with the introduction of transgenic te- nologies and nuclear cloning. Basic knowledge and techniques developed for lower vertebrate and invertebrate systems have facilitated the study of higher vertebrates, including humans. Many experiments that have first been performed on lower vertebrates provided the tools and strategies that could later be applied to other less readily available mammalian systems. The discovery of centrosomes in ascidians and sea urchin eggs now benefits studies of fertility and infertility in mammals. External in vitro fertilization, now a common technique in assisted fertilization, has only been possible as a result of numerous studies in lower systems in which external fertilization is natural. Egg activation, first explored in sea urchin and ascidian eggs, now benefits techniques designed to increase cl- ing efficien | 出版日期 | Book 2004 | 关键词 | Activation; DNA; cryopreservation; electron microscopy; genes; mammalian oocytes; reproducible techniques | 版次 | 1 | doi | https://doi.org/10.1385/1592597440 | isbn_softcover | 978-1-61737-374-9 | isbn_ebook | 978-1-59259-744-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 2004 |
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