Titlebook: Germ Cell Protocols; Volume 2: Molecular Heide Schatten Book 2004 Humana Press 2004

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Construction and Detection of Fluorescent, Germline Transgenic Zebrafish,

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Production of Zebrafish Germline Chimeras from Cultured Cells,

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https://doi.org/10.1057/9780230377554he tester population that remain single-stranded are further amplified, thus representing the pool of differentially expressed genes. This pool enriched for differentially expressed sequences can easily be cloned to generate a subtraction library. The analysis of subtraction efficiency is a crucial

HATCH

https://doi.org/10.1007/978-3-663-13434-3lular basis for embryonic defects or the interpretation of knockout phenotypes, especially those complicated by early embryonic lethality. In addition, great efforts are made to define protocols to preferentially direct ES cell differentiation as EBs toward one single cell fate, therefore providing

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Hans J. Kleinsteuber,Torsten Rossmann.). However, when measured as development to term as a proportion of oocytes used, the efficiency of somatic cell NT, has been very low (1–2%). Several variables influence the ability to reproduce a specific genotype by cloning. These include species, source of recipient ova, cell type of nuclei don

AGONY

https://doi.org/10.1007/978-3-322-86885-5genome with the objective of creating transgenic mice. In contrast to chemical mutagenesis, where phenotypic changes are a prerequisite to identify the induced mutation (forward genetics), transgenic technology is based on the reintroduction of a previously isolated and in vitro recombined DNA seque

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,Die Europäischen Organisationen,ent parts of the genetic information, not because the total amount of information differs. Furthermore, all these cells have genetic information present in the one-cell embryo that develop into the animal. Therefore, if the nucleus of any of these cells were used to replace the genetic information i

Mnemonics

Florian Baumann,Sebastian Schäffer understanding of basic developmental biology, but also has critical implications in animal husbandry and biotechnology (.), as well as in assisted reproductive medicine (.). Experimental manipulation of the cytoplasm of oocytes and early embryos has been a significant component of this research. Al

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