Titlebook: Genotyping; Methods and Protocol Stefan J. White,Stuart Cantsilieris Book 2017 Springer Science+Business Media New York 2017 DNA.Taqman-bas

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书目名称Genotyping影响因子(影响力)




书目名称Genotyping影响因子(影响力)学科排名




书目名称Genotyping网络公开度




书目名称Genotyping网络公开度学科排名




书目名称Genotyping被引频次




书目名称Genotyping被引频次学科排名




书目名称Genotyping年度引用




书目名称Genotyping年度引用学科排名




书目名称Genotyping读者反馈




书目名称Genotyping读者反馈学科排名

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Genotyping DNA Variants with High-Resolution Melting Analysis,destructive closed tube assay; after PCR, DNA melting can directly be performed on the amplified samples without any purification or separation steps. For single SNP genotyping, HRMA is an attractive alternative to Sanger sequencing, restriction enzyme analysis, and hydrolysis probes.

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In Situ Single-Molecule RNA Genotyping Using Padlock Probes and Rolling Circle Amplification,ys rely on whole specimen extracts, where heterogeneous spatial context of the specimen is lost. This chapter describes an up-to-date protocol for multiplexed, in situ genotyping of RNA in preserved tissue and cell lines, using padlock probes and rolling circle amplification. The presented approach

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,The MassARRAY® System for Targeted SNP Genotyping, The use of genome-wide SNP genotyping arrays has become increasingly more commonplace for gene discovery. However, smaller-scale genotyping platforms capable of efficiently genotyping tens to hundreds of SNPs are still crucial for many aspects of this work, including replication of associations. Th

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Targeted Capture and High-Throughput Sequencing Using Molecular Inversion Probes (MIPs),cing of genomic DNA. Several thousand genomic targets can be selectively captured using long oligonucleotides containing unique targeting arms and universal linkers. The ability to append sequencing adaptors and sample-specific barcodes allows large-scale pooling and subsequent high-throughput seque

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Analyzing Copy Number Variation Using Pulsed-Field Gel Electrophoresis: Providing a Genetic Diagnos4. In unaffected individuals the number of 3.3 kb D4Z4 units varies between 8 and 100, whereas 1–10 units are seen in FSHD1 cases. A homologous and heterogenous D4Z4 array can be found on chromosome 10q, but contractions of this array are typically not associated with FSHD. Discriminating between th

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Analysis of Copy Number Variation Using the Paralogue Ratio Test (PRT),ource of genetic variation within species. However, reliably determining copy number of a particular DNA sequence for a large number of samples can be challenging. Here, I describe and review the paralogue ratio test (PRT) in detail. PRT was developed to robustly type the CNV of the beta-defensin lo

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Genotyping Multiallelic Copy Number Variation with Multiplex Ligation-Dependent Probe Amplificationers of specific genes have been associated with different diseases. Precise genotyping of these loci can be complicated, and relies on accurate assays. Multiplex ligation-dependent probe amplification (MLPA) is a PCR-based approach that allows copy number determination of up to 50 genomic loci in a

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Analysis of Multiallelic CNVs by Emulsion Haplotype Fusion PCR,s very numerous small reaction chambers in which different PCR products from a single genomic DNA molecule are condensed into short joint products, to unite sequences in . from widely separated genomic sites. These products can therefore provide information about the arrangement of sequences and var