Titlebook: Genomic Mosaicism in Neurons and Other Cell Types; José María Frade,Fred H. Gage Book 2017 Springer Science+Business Media LLC 2017 FISH.n

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Giant Cell Reparative Granulomation, some populations of vertebrate neurons can also show double the normal amount of DNA, a condition referred to as somatic tetraploidy. These neurons are generated during early stages of development, as they migrate to their adult locations in the adult nervous system, and constitute subpopulati

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Poor-Prognosis Germ Cell Tumoursution of neurons with DNA content variation (DCV) in the context of preserved tissue architecture. The method had been optimized for DNA quantification of identified neurons but could easily be adapted to other tissues. It had been validated against chromogenic in situ hybridization (CISH) with chro

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Steven P. Rowe,Yafu Yin,Michael A. Gorins to isolate neuronal nuclei from human brain and identify megabase-scale copy number variants (CNVs) in single nuclei. The approach detailed herein includes use of CellRaft technology for single-nucleus isolation, the PicoPLEX approach to whole-genome amplification and library preparation, and a po

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R. Curtis Ellison,Peter F. Cohned to examine the genome at single-cell resolution. Here we describe the recent progress in the development of single-cell whole-genome amplification methods and the state of art for analyzing one of the major forms of genomic variations—copy number of variations (CNVs). Robust detection of CNVs in

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G. Schley,W. Hengstebeck,K. D. Bockwn to cause particular neurodegenerative diseases. We developed a dNTP-limited, competitive PCR technique to identify relative copy number differences between a reference and one or more target genes. Suitable fragments with single melting domains, well-separated melting temperatures, and no common

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