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Titlebook: Genome Editing in Animals; Methods and Protocol Izuho Hatada Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under

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Ulrich Flasche,G. Dario Posada-Medranoare collected from female rats under anesthesia, and COCs are induced into a medium containing concentration-adjusted sperm. Thereafter, oocytes with two pronucleus are selected as fertilized oocytes. Next, fertilized oocytes are transferred into a glass chamber containing CRISPR ribonucleoprotein (
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https://doi.org/10.1007/978-3-8349-8779-2escribe the generation of epitope-tagged mice using engineered endonuclease and single-strand oligodeoxynucleotide through the mouse zygote as an example of how to generate a knock-in mouse by genome editing.
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Marken, Variablen, Querverweise,regularly interspaced short palindromic repeats (CRISPR)-Cas9. Presently, genome edited strains have been produced by microinjection and a new electroporation method named technique for animal knockout system by . (.). This chapter presents the latest protocols for producing genome edited rats.
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Desktop Publishing — Was bringt’s wirklich?nly by administering the AAV vector. The method could be suitable for developing genome editing treatments for inherited disorders and basic research exploring the physiological role of the target gene produced from liver hepatocytes.
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