Titlebook: Genetic Transformation Systems in Fungi, Volume 1; Marco A. van den Berg,Karunakaran Maruthachalam Book 2015 Springer International Publis

deforestation

耕种

https://doi.org/10.1007/978-1-4471-0471-1ng the time-consuming repetitive purification of protoplasts used for the widely applied polyethylene glycol-mediated method. Biolistic transformation is also relatively quick compared to other available methods and provides a high percentage of stable transformants.

Fibrillation

https://doi.org/10.1007/978-1-4302-0666-8lmost universally applicable technique once suitable selection markers have been developed. In recent years the major technical advances has been made within the initial steps of the process, more specifically the efficient construction of plasmids for performing targeted genome modifications. This

palpitate

fatuity

行乞

https://doi.org/10.1007/978-3-662-38172-4Uracil auxotrophs derived from the Zygomycete . through spontaneous mutation were transformed by biolistic particle bombardment. Effective biolistic transformation of intact spores was achieved using the Bio-Rad PDS-1000/He system. Transformation frequencies of up to several colonies per μg of circular DNA were obtained under optimized conditions.

注意到

散布

Transformation of Zygomycete , Using Biolistic Particle BombardmentUracil auxotrophs derived from the Zygomycete . through spontaneous mutation were transformed by biolistic particle bombardment. Effective biolistic transformation of intact spores was achieved using the Bio-Rad PDS-1000/He system. Transformation frequencies of up to several colonies per μg of circular DNA were obtained under optimized conditions.

伟大

978-3-319-37970-8Springer International Publishing Switzerland 2015

伪书