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Titlebook: Genetic Engineering of Microorganisms for Chemicals; Alexander Hollaender,Ralph D. DeMoss,Ralph S. Wolf Book 1982 Springer Science+Busines

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书目名称Genetic Engineering of Microorganisms for Chemicals
编辑Alexander Hollaender,Ralph D. DeMoss,Ralph S. Wolf
视频video
丛书名称Basic Life Sciences
图书封面Titlebook: Genetic Engineering of Microorganisms for Chemicals;  Alexander Hollaender,Ralph D. DeMoss,Ralph S. Wolf Book 1982 Springer Science+Busines
描述The normal course of most biologically catalyzed processes is tightly regulated at the genetic and physiological levels. The regulatory mechanisms are diverse, sometimes redundant, and it is becoming increasingly apparent that, at the genetic level, the range of mechanisms may be limited only by the permutations and combina­ tions available. For each microbial cell, evolution appears to have resulted in maximized advantage to that cell, achieving regulatory balance. Genetic engineering encompasses our attempts to perturb the genetic regulation of a cell so that we may obtain desired other than normal outcomes, such as increased product formation, or new product formation. Following the groundwork established by a preceding symposium (Trends in the Biology of Fermentations for Fuels and Chemicals, Brookhaven National Laboratory, December 1980), the initial planning for this conference envisioned the juxtaposition of molecular genetic expertise and microbial biochemical expertise. The resultant interaction should encourage new and extended ideas for the improve­ ment of strains and for the generation of new regulatory combina­ tions to enhance microbial chemical production from cheap
出版日期Book 1982
关键词Fermentation; biochemistry; chemistry; genetic engineering; physiology; reaction
版次1
doihttps://doi.org/10.1007/978-1-4684-4142-0
isbn_softcover978-1-4684-4144-4
isbn_ebook978-1-4684-4142-0
copyrightSpringer Science+Business Media New York 1982
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Cloning Vectors Derived from Bacterial Plasmids,e number of naturally occuring plasmids has led to the conclusion that they have evolved through a process which can be referred to as “modular evolution”. In this process, various functional units (such as those involved in conjugal transfer, antibiotic resistance and colicin production) have been
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Expression of a Foreign Procaryotic Gene in ,,nsformation has lead to the development of a circular genetic map, the identification and approximate localization of the replication origin and terminus for the chromosome, as well as to studies of extrachromosomal inheritance (1,2). In recent years gene cloning in .. . has been accomplished by sev
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Fusion of Microbial Protoplasts: Problems and Perspectives, in the scheme of Figure 1. The rigid cell wall of bacteria, fungi, yeast, and algae (as well as of plant cells) can be removed without influencing the viability and integrity of the cell. The wall-less microorganism is surrounded by a plasma membrane, in hypertonic media it is spherical in shape, a
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Strategies: Models and Problems,before any engineering is begun, we might take note of microbial enzymatic reactions at present in operation. What general patterns have been established by the more subtle pressures of evolution? Time is not available for an adequate survey, and a few examples from one important area must suffice:
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Transformation of , Utilizing Recombinant Plasmid DNA, dependent on two different though related matters: an efficient transformation system for the organism and an appropriate vector. In addition, transformation in any organism involves two processes: a system which permits the uptake of DNA into the cell and the subsequent integration of the DNA into
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