Titlebook: Genetic Engineering in Eukaryotes; Paul F. Lurquin,Andris Kleinhofs Book 1983 Springer Science+Business Media New York 1983 ethics.food.ge

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Transfer of DNA into Higher Eukaryotic Cells Using Recombinant Vectors Based on Simian Virus 40 andods for introduction of DNA into mammalian cells ., more sensitive assays for vector function ., and new selectable markers for stable transformation of tissue culture cells .. In this article we present recent work from our laboratory relating to these areas.

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Construction and Transfer into Mammalian Cells of a Vector Containing Insect Histone Genes,to many types of eukaryotic cells, a revolution has occurred in the level of our understanding of the way genes are regulated in living cells. Nonetheless, in spite of the impressive progress made concerning the role of DNA sequence in functions such as promotion, enhancement, initiation of transcri

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An Insect Virus for Genetic Engineering: Developing Baculovirus Polyhedrin Substitution Vectors,ent (Miller, 1981a). Among the features which make baculoviruses highly advantageous as recombinant DNA vector systems are (1) a covalently-closed, circular, nuclear-replicating DNA genome, (2) an extendable rod-shaped capsid, (3) a group of genes, involved in occlusion, that are nonessential for in

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Bacterial-Plant Gene Cloning Shuttle Vectors for Genetic Modification of Plants, This requirement can be achieved by the development of systems for the delivery and integration of foreign genes into the plant genome. Two potential gene delivery systems have been the focus of considerable attention: the Ti plasmid of . and the DNA plant virion cauliflower mosaic virus (CaMV).. A

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Construction of Recombinant Ti Plasmids Containing the ,M Gene from ,. ,, which presently exist can be divided into two categories: 1) vectors which integrate within the host genome and 2) vectors which can be established as independent replicons in plant cells. The first category includes the T-DNA from . and the T-DNA from .. The second class of vectors comprises the c

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