Titlebook: Genetic Engineering; Principles and Metho Jane K. Setlow Book 1989 Plenum Press, New York 1989 genetic engineering.Tree Biology

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The Application of Antisense RNA Technology to Plants,ng. Gene expression can be inhibited in this manner; such regulation occurs naturally in bacteria and has been demonstrated experimentally in eukaryotes (for reviews see 1–6). Until recently, work in eukaryotes has utilized organisms such as . (7), . (8), . (9), and mammals, including cell lines of

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The Molecular Genetics of Plasmid Partition: Special Vector Systems for the Analysis of Plasmid Parwever, naturally occurring plasmids are very stably maintained in populations of the host bacteria. Thus, the individual copies must be distributed within the dividing cell in such a way as to ensure that each daughter cell receives at least one copy. This can be termed plasmid partition. A simple w

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DNA-Mediated Transformation of Phytopathogenic Fungi,est trees world-wide (1,2). The some 8,000 known phytopathogenic fungal species are distributed in all major taxonomic groups of fungi and display diverse physiological behaviors and life styles ranging from obligate parasites to facultative species (3). Some major fungal pathogens and their notable

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Fate of Foreign DNA Introduced to Plant Cells,velopment of . as a gene carrier to plant cells substantially stimulated the progress of experimental plant molecular biology and also the development of alternatives to . for gene transfer to plants. The construction of hybrid selectable marker genes and the establishment of clean selection systems

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Generation of cDNA Probes by Reverse Translation of Amino Acid Sequence,A correct selection of the oligonucleotide probe is difficult since the genetic code is degenerate (2). Oligonucleotide probes lacking complementarity to their target sequences generate spurious hybridization signals and fail to identify authentic clones, rendering cDNA library screening a complicat

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Molecular Genetics of Self-Incompatibility in Flowering Plants, inbreeding. As the male and female sexual organs, the anthers and the pistil, are often arranged on the same plant in close physical proximity, the potential for self-mating, in the absence of specific mechanisms for its prevention, is high. A formal definition of self-incompatibility is given by L