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楼主: Causalgia
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Theoretical and Computer Analysis of Protein Primary Sequences: Structure Comparison and Predictionmined nucleotide sequence and are now wondering which theoretical methods and associated computer programs you should use to glean structural and functional information from the mere sequence of letters.”
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Applications of the Firefly Luciferase as a Reporter Gene,portant tools in the arsenal of techniques used to analyze gene regulation are reporter genes, which, when linked to appropriate regulatory elements, provide insights into the levels of gene expression and the DNA segments that modulate this expression. In addition, such genes may also be utilized t
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Phosphorothioate-Based Oligonucleotide-Directed Mutagenesis,his situation has changed drastically with the advent of automated methods of oligonucleotide synthesis in combination with rapid sequencing and cloning techniques (2). Now, with the technique of oligonucleotide-directed mutagenesis specific and direct manipulations at the level of a single base in
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Design and Use of , Transformation Vectors,icultural biotechnology. The cause of this interest is the soil bacterium . and its complex relationship with plant cells. In this review we will describe both the interaction of . with plant cells which leads to DNA transfer and integration into the nuclear genome, and how an understanding of this
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Cell Commitment and Determination in Plants,rmination (1). In spite of its importance -- E. B. Wilson (2) called it “the fundamental problem...which includes all others” -- determination has received far less attention from molecular biologists than has cell differentiation. One reason may be that determination is not a visible property of ce
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