Titlebook: Genetic Engineering; Principles and Metho Jane K. Setlow Book 2000 Kluwer Academic/Plenum Publishers 2000 cloning.evolution.gene expression

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Targeted Gene Repair in Mammalian Cells Using Chimeric Oligonucleotides,A synthesis and change developmental progression (1), while in other cases more permanent alteration at the chromosomal level has been attempted (2). These types of approaches seek not to replace traditional protocols of gene knock-out, gene knock-in or gene replacement, but rather augment them, add

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Exploring the Mechanism of Action of Insecticidal Proteins by Genetic Engineering Methods,s has revolutionized pesticide application. The impact of this new technology has already been touched upon in this series (1–5). To a large extent this has been possible because of the extensive basic research that has been done on the . (Bt) Cry proteins (5,6). The following review will provide an

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ET-Cloning: Think Recombination First,y. The most commonly used way to engineer DNA relies on the ligation of two individual DNA molecules with the use of restriction sites. Although useful, this method has some limitations which makes it elaborate or even inapplicable for many DNA manipulations. Firstly, it relies on the presence of su

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,Growth and Genetic Modification of Human β-Cells and β-Cell Precursors,mplish this, the ß-cell must sense the concentration of blood glucose in order to release the right amount of insulin rapidly. While mechanical devices to accomplish this are being developed, ultimately the best approach is likely to be based on genetic modification of cells. Because of the complex

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Elucidation of Biosynthetic Pathways by Retrodictive/Predictive Comparison of Isotopomer Patterns Dobel Prize was awarded to Melvin Calvin. On the other hand, it is important to note that the biosynthetic literature is replete with incorrect conclusions derived from isotopic studies. To give just one example, numerous plant terpenes have been claimed incorrectly to be biosynthesized . the mevalon

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Are Gene Silencing Mutants Good Tools for Reliable Transgene Expression or Reliable Silencing of Enr proteins adding a new structural or regulatory property to the plant, ii) introduction of antisense transgenes that encode RNA that interferes with the expression of endogenous genes or gene families. Many successful achievements involving transgenic plants expressing new traits have been obtained

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Manipulating Plant Viral RNA Transcription Signals,ycle. Consequently, RNA viruses do not transcribe in the classical sense. However, many RNA viruses make partial copies or transcripts from their RNA genomes that serve primarily as translational templates. This process is termed viral RNA transcription. This chapter will discuss the most recent fin

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Telomerase and Cancer,ed laboratory cell lines which have been proliferating in culture for decades [for example, (1)]. Clinically, immortality provides malignant cells with unlimited growth potential, a property that underlies the fatal nature of cancer. This capacity, specific to tumor cells, likely results from a numb