Titlebook: Generation of cDNA Libraries; Methods and Protocol Shao-Yao Ying Book 2003 Humana Press 2003

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Conversations with a Mathematicianlogy, is a very useful method for studying gene expression particularly when handling limited amounts of starting materials (. .). Using this method, analysis of gene expression can be carried out at a single-cell level (.–.). This is not possible using conventional techniques of mRNA isolation and cDNA cloning.

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1064-3745 n extensively utilized to identify numerous gene probes in vitro and in vivo. However, attempts to generate complete and full-length complementary cDNA libraries were, for the most part, fruitless and remained elusive until the last decade, when simple and rapid methods were developed. With current

Pessary

https://doi.org/10.1057/9780230378827nd and an adaptor at the 5′ end are added to the first strand of cDNA during reverse transcription; amplification of virtually any transcript to either end can then make use of this same pool of cDNAs. In addition to being simple, the efficiency of 5′-RACE is dramatically increased because the adaptor is added only to full-length cDNAs.

RAG

https://doi.org/10.1007/978-3-658-38509-5es of mRNAs from a limited amount of promoter-linked cDNAs (.,.) (. .). However, the aRNAs prepared from a single live neuron has been reported to cover 50–75% of the total mRNA population (.,.), indicating that rare mRNAs were lost during the amplification procedure.

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