Titlebook: Gene Transfer and Expression Protocols; E. J. Murray Book 1991 Springer Science+Business Media New York 1991

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Preparation of High-Molecular-Weight DNA for Use in DNA Transfection,eory, for any phenotype for which there is a suitable selection in cell culture, a gene sequence encoding that phenotype can be transferred and will complement the selection. If the donor sequences are derived from a different species of origin, the presence of the donor DNA in the recipient cell ca

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Chromosome-Mediated Gene Thnsfer,n cells. This technique, termed chromosome-mediated gene transfer (CMGT), involves the transfer of subchromosomal fragments from the cells of one species (the donor, usually human) to those of another (the recipient, usually a rodent) that have the ability to maintain them. CMGT thus fills a gap bet

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