Titlebook: Gene Transfer and Expression; A Laboratory Manual Michael Kriegler Book 1990 Stockton Press 1990 Calcium.DNA.nucleic acid.protein.RNA

archaeology

老人病学

nuclear-tests

DNA Transfer (2) create DNA-containing complexes whose charge characteristics are compatible with DNA uptake by the target cell; or (3) result in the transient formation of pores in the plasma membrane of a cell exposed to an electric pulse, pores of sufficient size to allow DNA to enter the target cell.

热情赞扬

Expression Cloningis type include selectable markers and oncogenes, as well as cytokines (Wong et al., 1985) and membrane proteins including cell-surface antigens (Aruffo and Seed, 1987) and cellular receptors (D’Andrea et al., 1989).

MUTED

蒸发

ANIM

苦笑

Clinical Anatomy and Embryologylled incubator, an inverted microscope, a 4°C refrigerator, a −20°C freezer, and a liquid-nitrogen freezer for long-term cell storage. It is important that the flow hood, incubator, and microscope are located such that they are separate from the facilities used for the manipulation of bacterial or y

孵卵器

anachronistic

https://doi.org/10.1007/978-981-10-0240-3tion can be infected with a recombinant retrovirus. All other means of stable transformation are less efficient. To facilitate the isolation of cells stably transformed with the DNA of interest, a gene encoding a dominant selectable marker is usually included in the transfection protocol. Such a gen