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Titlebook: Gene Therapy Protocols; Volume 1: Production Joseph M. Le Doux Book 2008Latest edition Humana Press 2008 HIV.Virus.cell biology.chromosomal

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Methods for the Production of First Generation Adenoviral Vectors,ltured cells and for gene therapy where high-level, transient transgene expression is desired. This chapter describes in detail methods for the production and characterization of E1-deleted, first generation adenoviral vectors.
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Design of Trans-Splicing Adeno-Associated Viral Vectors for Duchenne Muscular Dystrophy Gene Theraptermine the optimal gene splitting site for the mini-dystrophin gene. We also provide methods to evaluate transduction efficiency of the mini-dystrophin .-splicing vectors in . mouse, a model for DMD.
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