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Titlebook: Gene Therapy Protocols; Volume 2: Design and Joseph M. Doux Book 20083rd edition Humana Press 2008 DNA.Promoter.Virus.gene expression.gene

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发表于 2025-3-21 16:44:51 | 显示全部楼层 |阅读模式
书目名称Gene Therapy Protocols
副标题Volume 2: Design and
编辑Joseph M. Doux
视频video
概述Collects time tested, reproducible laboratory protocols, including tricks and hints, written by experts in the field.Covers current and emerging methods for production of viral and non-viral gene tran
丛书名称Methods in Molecular Biology
图书封面Titlebook: Gene Therapy Protocols; Volume 2: Design and Joseph M. Doux Book 20083rd edition Humana Press 2008 DNA.Promoter.Virus.gene expression.gene
描述.In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this second volume of Gene Therapy Protocols: Design and Characterization of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression. This second volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical re
出版日期Book 20083rd edition
关键词DNA; Promoter; Virus; gene expression; gene therapy; gene transfer; gene transfer vectors; genes; tissue; tra
版次3
doihttps://doi.org/10.1007/978-1-60327-248-3
isbn_softcover978-1-62703-964-2
isbn_ebook978-1-60327-248-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2008
The information of publication is updating

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Scaleable Purification of Adenovirus Vectors,ity of purified virus required for these studies necessitate that purification methods must shift from classical density gradient ultracentrifugation to scaleable approaches. A methodology is described herein using batch centrifugation, tangential flow ultrafiltration, and chromatography to purify a
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Quantifying the Titer and Quality of Adenovirus Stocks,assays for the facile quantitation of adenoviral particles and the assignment of their infectious potency. The Genome Quantitation Assay (GQA) and the QPCR-Based Potency Assay (QPA) developed for adenoviruses offer the attributes of precision, rapidity, and high throughput either performed manually
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PEGylated Adenovirus for Targeted Gene Therapy,or. Modification of the surface of adenovirus with heterofunctional PEG allows further modification of the capsid with ligands. In addition, heterofunctional PEG modification ablates the normal tropism of the virus and reduces transduction of non-target tissues in vivo. Moreover, the addition of PEG
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