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Titlebook: Gene Quantification; François Ferré Book 1998 Birkhäuser Boston 1998 DNA.Expression.PCR.Pathogen.Polymerasekettenreaktion.Quantitative PCR

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发表于 2025-3-21 16:04:36 | 显示全部楼层 |阅读模式
书目名称Gene Quantification
编辑François Ferré
视频video
丛书名称Advanced Biomedical Technologies
图书封面Titlebook: Gene Quantification;  François Ferré Book 1998 Birkhäuser Boston 1998 DNA.Expression.PCR.Pathogen.Polymerasekettenreaktion.Quantitative PCR
描述Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo­ bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num­ bers based on the kinetics of the reassociation process - the so-called Cot analysis.
出版日期Book 1998
关键词DNA; Expression; PCR; Pathogen; Polymerasekettenreaktion; Quantitative PCR; RNA; Termination; biology; develo
版次1
doihttps://doi.org/10.1007/978-1-4612-4164-5
isbn_softcover978-1-4612-8682-0
isbn_ebook978-1-4612-4164-5
copyrightBirkhäuser Boston 1998
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Book 1998 ribosomal genes and the immunoglo­ bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num­ bers based on the kinetics of the reassociation process - the so-called Cot analysis.
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uch as the ribosomal genes and the immunoglo­ bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num­ bers based on the kinetics of the reassociation process - the so-called Cot analysis.978-1-4612-8682-0978-1-4612-4164-5
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