| 书目名称 | Gene Function Analysis | | 编辑 | Michael F. Ochs | | 视频video | | | 概述 | Brings together a number of techniques, both computational and biological, that have developed recently for looking at gene function.Contains Notes sections with troubleshooting guides.Easy-to-follow | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | This volume of Methods in Molecular Biology focuses on techniques to determine the function of a gene. Traditionally, the function of a gene was determined following cloning, which provided its DNA sequence and an ab- ity to modify this sequence. Experiments were performed that looked for p- notypic changes in a cell line or model organism following modifications to the sequence, knocking out of the gene, or enhancing expression of the gene. In the 1990’s, the growing sequence databases and the BLAST algorithm provided additional power by allowing identification of genes with known function that had similar sequences and potentially similar molecular mechanisms. On the experimental side, methods, such as two-hybrid screening that could directly determine the partners of specific proteins and even the domains of interaction, came into widespread use. With the advent of high-throughput technologies following completion of the human genome project and similar projects in model organisms, the n- ber of genes of interest has expanded and the traditional methods for gene fu- tion analysis cannot achieve the throughput necessary for large-scale exploration. Although computational tools su | | 出版日期 | Book 2007 | | 关键词 | DNA; Mutant; Promoter; Proteomics; Stefan Dübel; bioinformatics; gene expression; genes; genome; molecular bi | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-59745-547-3 | | isbn_softcover | 978-1-61737-748-8 | | isbn_ebook | 978-1-59745-547-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2007 |
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发表于 2025-3-21 19:04:04
