| 书目名称 | Gene Delivery to Mammalian Cells | | 副标题 | Volume 1: Nonviral G | | 编辑 | William C. Heiser | | 视频video | http://file.papertrans.cn/382/381911/381911.mp4 | | 概述 | Includes supplementary material: | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, and hydrodynamics. Each protocol contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo..Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers | | 出版日期 | Book 2004 | | 关键词 | DNA; Ethylen; Vivo; gene transfer; genes; liposomes | | 版次 | 1 | | doi | https://doi.org/10.1385/1592596495 | | isbn_softcover | 978-1-61737-349-7 | | isbn_ebook | 978-1-59259-649-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2004 |
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