Titlebook: Gene Correction; Methods and Protocol Francesca Storici Book 2014 Springer Science+Business Media, LLC 2014 gene correction.gene repair.gen

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https://doi.org/10.1007/978-3-642-76453-0affic Light Reporter system, which uses a flow cytometric assay to simultaneously detect both gene repair and mutagenic nonhomologous end-joining outcomes at a single targeted site in mammalian cells. With these methods, novel nickases can be designed and tested for use in gene correction with novel target sites.

利用

Design and Analysis of Site-Specific Single-Strand Nicking Endonucleases for Gene Correctionaffic Light Reporter system, which uses a flow cytometric assay to simultaneously detect both gene repair and mutagenic nonhomologous end-joining outcomes at a single targeted site in mammalian cells. With these methods, novel nickases can be designed and tested for use in gene correction with novel target sites.

RODE

https://doi.org/10.1007/978-3-0348-9389-3ns increasingly include data utilizing DT40 cells. In this chapter, we describe the current standard methods of multiple genetic manipulation in DT40 cells. We place a particular emphasis on describing the basic concepts and theoretical background of the genetic manipulation of DT40 cells for researchers who are new to such techniques.

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https://doi.org/10.1007/978-1-349-06540-0ome, which can be achieved by targeting genes by homologous recombination. This chapter will describe a protocol for gene modification of pluripotent stem cells by homologous recombination and several methods for the screening and identification of successfully modified clones.

Oafishness

Chemotherapy Protocols and Infusion Sequenceman genome. In this chapter, we describe methodology for engineering and characterizing chimeric . transposase enzymes, including experimental approaches for evaluating activity and targeting specificity in the human genome.

陶瓷

Multiple Genetic Manipulations of DT40 Cell Linens increasingly include data utilizing DT40 cells. In this chapter, we describe the current standard methods of multiple genetic manipulation in DT40 cells. We place a particular emphasis on describing the basic concepts and theoretical background of the genetic manipulation of DT40 cells for researchers who are new to such techniques.

展览

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RecTE,-Mediated Recombineering in ,get location. Cells transformed with the substrate undergo homologous recombination between the genomic DNA and the recombineering substrate. The recombinants are found by selection for traits carried by the recombineering substrate, usually antibiotic resistance.

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Genome Manipulations with Bacterial Recombineering and Site-Specific Integration in Drosophilaineering to conduct targeted modifications of individual loci. Here we describe the recombineering designs and procedures for the introduction of epitope tags, in-frame deletion mutations, and point mutations into plasmids that can later be used for SIRT.