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Titlebook: Gapmers; Methods and Protocol Toshifumi Yokota,Rika Maruyama Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 A

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书目名称Gapmers
副标题Methods and Protocol
编辑Toshifumi Yokota,Rika Maruyama
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Gapmers; Methods and Protocol Toshifumi Yokota,Rika Maruyama Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 A
描述.This volume presents a comprehensive collection of detailed state-of-the-art protocols for gapmer-mediated RNA knockdown from leaders in the field. Chapters detail a historical and contemporary perspective of RNase H-inducing antisense oligonucleotides called gapmers and gapmer-mediated RNA knockdown for basic research and therapies. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... Authoritative and cutting-edge, .Gapmers: Methods and Protocols. aims to provide diverse applications of gapmers along with protocols that will assist readers in moving the frontier..
出版日期Book 2020
关键词Antisense oligonucleotides; Gene silencing; Drug development; Gapmer therapeutics; Gapmer-mediated degra
版次1
doihttps://doi.org/10.1007/978-1-0716-0771-8
isbn_softcover978-1-0716-0773-2
isbn_ebook978-1-0716-0771-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
The information of publication is updating

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Gapmers978-1-0716-0771-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Piero Filipponi,Alwyn F. Horadamtudy estimates that at least four times more lncRNAs are typically present than coding RNAs in humans. However, function of more than 95% of human lncRNAs are still unknown. Synthetic antisense oligonucleotides called gapmers are powerful tools for lncRNA loss-of-function studies. Gapmers contain a
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Some Notes on Fibonacci Binary Sequences,ogenicity, thereby providing considerable insight to develop suitable therapies. With the successful translation of antisense oligonucleotides (AOs) from in vitro into animal models and clinical practice, modifications are being continuously made to the AOs to improve the pharmacokinetics and pharma
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Computing with the Unpredictablef repeat sequences in the . and . genes, respectively. The expansions are highly unstable and biased for further expansion in somatic cells and across generations. Despite the different genes involved, DM1 and DM2 share several clinical features due to having the similar underlying mechanism of repe
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Interdisciplinary Systems ResearchRNA (cRNA) strand. HDOs were originally designed to improve the properties of RNase H-dependent ASOs and we reported in our first paper that HDOs conjugated with an α-tocopherol ligand (Toc-HDO) based on a gapmer ASO showed 20 times higher silencing effect to liver apolipoprotein B (apoB) mRNA in vi
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