Titlebook: Gap Junction Protocols; Mathieu Vinken,Scott R. Johnstone Book 2016 Springer Science+Business Media, LLC, part of Springer Nature 2016 Con

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Gap Junction Protocols978-1-4939-3664-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerasewell-established reverse transcription quantitative real-time polymerase chain reaction procedure optimized for analysis of hepatic connexins. The method includes RNA extraction and subsequent quantification, generation of complementary DNA, quantitative real-time polymerase chain reaction, and data analysis.

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DNA Methylation Analysis of Human Tissue-Specific Connexin Genes,e DNA methylation-specific loci of connexin genes. This chapter describes analysis of selective connexin32 and connexin43 gene DNA methylation in human gastric tissues using methylation-specific PCR, bisulfite-specific PCR sequencing as well as MassArray techniques.

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