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Titlebook: Galectins; Methods and Protocol Sean R. Stowell,Richard D. Cummings Book 2015 Springer Science+Business Media New York 2015 galectin funct

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发表于 2025-3-21 19:21:08 | 显示全部楼层 |阅读模式
书目名称Galectins
副标题Methods and Protocol
编辑Sean R. Stowell,Richard D. Cummings
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Galectins; Methods and Protocol Sean R. Stowell,Richard D. Cummings Book 2015 Springer Science+Business Media  New York 2015 galectin funct
描述.Galectins: Methods and Protocols .is the first book solely dedicated to methodological approaches designed to study galectin function. The galectin family represents one of the most pleiotropic families, with individual members having been implicated in various aspects of nearly every biological process described, from RNA splicing to complex regulatory circuits that orchestrate adaptive immunity. Given the diverse roles of galectins in a variety of biological systems, studying these glycan binding proteins often requires the assimilation of diverse technical skills to fully appreciate their biological function.  Their nearly ubiquitous expression and ability to bind highly modifiable carbohydrate ligands, in addition to a variety of other regulatory proteins, allows these glycan binding proteins (GBPs) to possess the capacity to regulate a wide variety of biological processes. Individual chapters are dedicated to examining salient features of galectin functions. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocol
出版日期Book 2015
关键词galectin function; galectins; glycan-binding proteins; mass spectrometric mapping; microarrays
版次1
doihttps://doi.org/10.1007/978-1-4939-1396-1
isbn_softcover978-1-4939-5296-0
isbn_ebook978-1-4939-1396-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

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Examination of Galectins in Phagocytosis, as an example. We emphasize the function of endogenous galectin-3 as determined by comparison of phagocytosis by macrophages from galectin-3 knockout mice and wild-type mice. We focus on the role of galectin-3 in phagocytosis of pathogens and Fcγ receptor-mediated phagocytosis of opsonized cells and particles.
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Examination of the Role of Galectins in Intestinal Inflammation,m with the research of intestinal inflammation may be the requirement of some unique techniques. Therefore, we herein describe how to induce intestinal inflammation and how to isolate lymphocyte, myeloid cell, follicular cell, and epithelial cell populations separately from the intestine for the study of intestinal inflammations.
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Examination of the Role of Galectins and Galectin Inhibitors in Endothelial Cell Biology, vessels. Galectins are involved in several of these activities. In this chapter we describe methods to study galectins and galectin inhibition in three key functions of endothelial cells during angiogenesis, i.e., endothelial cell migration, endothelial cell sprouting, and endothelial cell network formation.
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,Inertial Force Sensors — Accelerometers,mation regarding GBP–glycan interactions, but have also resulted in significant insight into the binding specificity and biological activity of the galectin family. We will describe the methods used when employing glycan microarray platforms to examine galectin–glycan binding specificity and function.
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