Titlebook: G Protein-Coupled Receptor Screening Assays; Methods and Protocol Sofia Aires M. Martins,Duarte Miguel F. Prazeres Book 2021Latest edition

phytochemicals

博识

Luciferase Complementation Approaches to Measure GPCR Signaling Kinetics and Bias,r kinetic properties. Recent developments in complementation approaches, based on a small bright shrimp luciferase Nanoluc, provide a new route to kinetic analysis of GPCR signaling in living cells that is amenable to the throughput required for compound profiling. In the NanoBiT luciferase compleme

护航舰

https://doi.org/10.1007/978-3-8348-8201-1 state and upon receptor agonism, and the evaluation of the biological functions of the GPCR in appropriate cell lines. The lack of standardized methodologies to study lipid rafts, in general, and of the workings of GPCRs in lipid rafts, in particular, and the inescapable drawbacks of current method

描述

https://doi.org/10.1007/978-3-8348-1976-5maximize sensitivity while minimizing noise. These methods may also be applicable for assessing the expression of members of families of other low abundance genes via high-throughput analyses of mRNAs, followed by independent confirmation and validation of results via qPCR.

CBC471

Obligatory

ENACT

Migratory

https://doi.org/10.1007/978-3-322-91961-8detecting G protein activation monitor the generation of second messengers ([Ca.]., cAMP, IP.) or active G proteins (with GTP-g-S for instance). While these approaches have proven sensitive and robust, they are not suited for the detection of a single GPCR-G protein interaction. Here, we present in

CAPE

关心

Book 2021Latest editionown pitfalls. .Authoritative and up-to-date, .G Protein-Coupled Receptor Screening Assays: Methods and Protocols, Second Edition. aims to provide the tools necessary to contribute to the advancement of GPCR research and discovery and ultimately lead to the availability of innovative and more efficie