书目名称 | Fundamentals of Fluorescence Microscopy | 副标题 | Exploring Life with | 编辑 | Partha Pratim Mondal,Alberto Diaspro | 视频video | | 概述 | A comprehensive text that starts from the fundamental optical imaging concepts.Guides the readers to the most advanced methods in fluorescence imaging and super-resolution techniques.An essential text | 图书封面 |  | 描述 | This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. .The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These fact | 出版日期 | Textbook 2014 | 关键词 | Advanced fluorescence microscopy techniques; Advances in fluorescence spectroscopy and microscopy; Flu | 版次 | 1 | doi | https://doi.org/10.1007/978-94-007-7545-9 | isbn_softcover | 978-94-017-7966-1 | isbn_ebook | 978-94-007-7545-9 | copyright | Springer Science+Business Media Dordrecht 2014 |
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