书目名称 | Functional Proteomics | 副标题 | Methods and Protocol | 编辑 | Julie D. Thompson,Marius Ueffing,Christine Schaeff | 视频video | | 概述 | Unique combination of reproducible laboratory protocols and state-of-the-art bioinformatics methods dedicated to functional proteomics.A comprehensive methodological compendium, covering sample prepar | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .As the emerging field of proteomics continues to expand at an extremely rapid rate, the relative quantification of proteins, targeted by their function, becomes its greatest challenge. Complex analytical strategies have been designed that allow comparative analysis of large proteomes, as well as in depth detection of the core proteome or the interaction network of a given protein of interest. In .Functional Proteomics: Methods and Protocols., expert researchers describe the latest protocols being developed to address the problems encountered in high-throughput proteomics projects, with emphasis on the factors governing the technical choices for given applications. The case studies within the volume focus on the following three crucial aspects of the experimental design: 1) the strategy used for the selection, purification and preparation of the sample to be analyzed by mass spectrometry, 2) the type of mass spectrometer used and the type of data to be obtained from it, and 3) the method used for the interpretation of the mass spectrometry data and the search engine used for the identification of the proteins in the different types of sequence data banks available. As a part of the | 出版日期 | Book 2008 | 关键词 | Complex functional systems; High-throughput proteomics; Mass spectrometry; Protein analysis; Protein exp | 版次 | 1 | doi | https://doi.org/10.1007/978-1-59745-398-1 | isbn_softcover | 978-1-61737-860-7 | isbn_ebook | 978-1-59745-398-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 2008 |
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