Titlebook: Epigenome Editing; Methods and Protocol Albert Jeltsch,Marianne G. Rots Book 2024Latest edition The Editor(s) (if applicable) and The Autho

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https://doi.org/10.1007/978-3-030-11470-1biochemical modifications of chromatin at almost any specific endogenous locus. Their locus-specificity unlocks unique information including the functional roles of distinct modifications at specific genomic loci. Given the growing interest in using these tools for biological and translational studi

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https://doi.org/10.1057/9781137506726 binding domains fused with effector domains, known as epi-editors. However, the constitutive expression of dCas9-based epi-editors presents challenges, including off-target activity and lack of temporal resolution. Recent advancements of dCas9-based epi-editors have addressed these limitations by i

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Misunderstanding International Relationsir individual domain’s function, both as modular parts and as full proteins. Transcriptional effector domains are one class of protein domains that regulate transcription and chromatin. These effector domains either repress or activate gene expression by interacting with chromatin-modifying enzymes,

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https://doi.org/10.1007/978-3-031-43571-3 are being incorporated into fusion proteins. Development of these fusion proteins, called epigenome editors, has outpaced the study of proteins that interact with edited chromatin. One type of protein that acts downstream of chromatin editing is the reader-effector, which bridges epigenetic marks w

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Mit ADHS vom Kindergarten in die Schule in the etiology of several diseases, such as cancer and imprinting diseases. Accordingly, technologies designed to manipulate DNA methylation at specific loci are considered worthwhile and many epigenome editing technologies have been developed, which were based on ZF, TALE, and CRISPR–dCas9. Here,

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Mit Atemübungen zum Gefühlsausdruckn, targeting . imprinted domain, a well-studied imprinted locus, in ES cells. In this protocol, plasmid vectors expressing the DNA methylation editing tools, combining the CRISPR/dCas9 system and the SunTag system coupled to a DNA methyltransferase or a TET enzyme, are introduced into cells for tran

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https://doi.org/10.1007/978-3-322-82692-3 epigenome editors to virtually any genomic site. This advancement in DNA-targeting technology brings allele-specific epigenome editing into reach, a “super-specific” variation of epigenome editing whose goal is an alteration of chromatin marks at only one selected allele of the genomic target locus