Titlebook: Extracellular Vesicles; Methods and Protocol Winston Patrick Kuo,Shidong Jia Book 2017 Springer Science+Business Media, LLC, part of Spring

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Winston Patrick Kuo,Shidong JiaIncludes cutting-edge techniques for the study of extracellular vesicles.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/319943.jpg

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Emil Salmo,Patrick O’Connolly,Najib Haboubimethods for isolating EVs from cell culture media, but the most popular methods involve purification based on ultracentrifugation. Here, we provide a detailed protocol for isolating EVs by differential ultracentrifugation and analyzing EV proteins (such as the tetraspanins CD9, CD63 and CD81) by western blotting.

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