Titlebook: Extracellular Vesicles; Methods and Protocol Winston Patrick Kuo,Shidong Jia Book 2017 Springer Science+Business Media, LLC, part of Spring

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Anthony Gonçalves,Patrice Viensis allows for cross platform comparison and the ability to monitor both quality control (QC) and quality assurance (QA). The method here describes the use of nanoparticles to optimize a flow cytometer for small particle detection. It also outlines the procedures necessary to recover EVs for downstream applications.

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Red Blood Cells: A Source of Extracellular Vesicles,a membrane. On the other hand, RBC EVs are generated during normal aging of RBCs in circulation by budding of the plasma membrane due to complement-mediated calcium influx, followed by vesicle shedding. This makes red blood cells and stored red cells a reliable source of EVs for basic and clinical research.

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Paper-Based for Isolation of Extracellular Vesiclesred EVs can be assessed using scanning electron microscopy (SEM), transcriptome analysis, and paper-based enzyme-linked immunosorbent assays (pELISA), respectively. A colorimetric readout can be detected from 10 μL serum within 10 min.

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