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Titlebook: Extracellular Matrix Protocols; Charles H. Streuli,Michael E. Grant Book 20001st edition Springer Science+Business Media New York 2000

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发表于 2025-3-21 19:30:40 | 显示全部楼层 |阅读模式
书目名称Extracellular Matrix Protocols
编辑Charles H. Streuli,Michael E. Grant
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Extracellular Matrix Protocols;  Charles H. Streuli,Michael E. Grant Book 20001st edition Springer Science+Business Media New York 2000
描述It is now widely accepted that much of the dynamic function of cells and tissues is regulated from outside the cell by the extracellular matrix. In ad- tion to its conventional role in providing a scaffold for building tissues, the extracellular matrix acts as a directional highway for cellular movement and provides instructional information for promoting survival, proliferation, and differentiation. Indeed, the extracellular matrix is beginning to take a starring role in the choreography of cell and tissue function. The diverse roles of the extracellular matrix are reflected in its highly complicated structure, consisting of an ever increasing number of components. Yet the mechanisms of extracellular matrix assembly and how they influences cell behavior are only just beginning to be understood. In order to solve these problems new methodologies are, of necessity, being developed. Many of these technologies are highly sophisticated and are currently available only in a ha- ful of laboratories. However, we believe that they can readily be transported and established by other researchers. Thus, the purpose of Extracellular Matrix Protocols is to present some of these complicated tech
出版日期Book 20001st edition
版次1
doihttps://doi.org/10.1385/1592590632
isbn_ebook978-1-59259-063-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2000
The information of publication is updating

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Quantitative Determination of Collagen Crosslinkse stabilized to provide mechanical strength by a series of intermolecular crosslinks. These links are formed by oxidative deamination of the ε-amino group of the single lysine in the amino and carboxy-telopeptides by lysyl oxidase. The aldehyde thus formed reacts with an ε-amino group of a lysine at
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Analysis of Laminin Structure and Function with Recombinant Glycoprotein Expressed in Insect Cellsctivities in basement membrane glycoproteins. This has been particularly valuable in the case of laminins where the relationship between structure and function has been difficult to establish. Several characteristics of the laminins lie at the heart of the problem. First, the molecules are very larg
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Recombinant Collagen Trimers from Insect Cells and Yeast their isolation for characterization at the protein level has so far been impossible. Some of the fibril-forming collagens are now in medical use, in applications ranging from the use of type I collagen as a biomaterial and a delivery system for several drugs to trials for the potential of type II
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Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Str advantages. First, eukaryotic expression can be expected under optimum conditions to produce a large proportion of correctly folded molecules. ECM proteins are made from a group of 25 structurally known (.) and about 200 cDNA derived domains many of which regularly reappear in the different protein
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Preparation of Isotopically Labeled Recombinant Fragments of Fibronectin for Functional and Structurtructure, and function. However, their primary structures have revealed that their polypeptide components are frequently comprised of a series of sequence repeats or “modules” (.). This mosaic nature of ECM macromolecules permits their properties to be analyzed by a “dissection” strategy (.), where
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Reconstitution of Functional Integrin into Phopsholipid Vesicles and Planar Lipid Bilayersion, cell migration, proliferation, differentiation, and gene expression (.,.). Integrins in their native environment can diffuse in the plane of the membrane, and this mobility is required for processes like the assembly of integrins to focal contacts or cell migration (.,.).
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Confocal-FRAP Analysis of ECM Molecular Interactions). It is the ECM that defines the architecture, the form, and the biomechanical properties of different tissues (.,.). Among the nonfibrillar macromolecules, the highly charged proteoglycans and hyaluronan are major components that occur at high concentration and greatly influence the movement of so
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Atomic Force Microspopy Measurements of Intermolecular Binding Strengthining their functional contribution to the maintenance of the anatomical integrity of the adult and embryonal multicellular organism, to fertilization, to blood cell adhesiveness in normal and pathological conditions, to tumor cell adhesion, to parasite-host interactions, and to cellular association
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