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Titlebook: Extracellular Matrix; Jennie B. Leach,Elizabeth M. Powell Book 2015 Springer Science+Business Media New York 2015 Bioengineering.Cell-extr

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书目名称Extracellular Matrix
编辑Jennie B. Leach,Elizabeth M. Powell
视频video
概述Includes cutting-edge methods and protocols involving the extracellular matrix in native, injured, and engineered neural tissues.Provides step-by-step detail for the utmost clarity.Contains some key i
丛书名称Neuromethods
图书封面Titlebook: Extracellular Matrix;  Jennie B. Leach,Elizabeth M. Powell Book 2015 Springer Science+Business Media New York 2015 Bioengineering.Cell-extr
描述.This volume provides comprehensive procedures for analyzing the extracellular matrix in native, injured, and engineered neural tissues. Divided into four parts, each focusing on different aspects of the extracellular matrix and the nervous system, .Extracellular Matrix. covers methods to analyze native tissue, in vitro models for investigating cell-extracellular matrix interactions in a variety of contexts, protocols to investigate the role of the extracellular matrix in nervous system injury, degeneration, and regeneration, as well as therapeutics and engineered systems. Each chapter is written by leading experts and presents established protocols in a concise format, encompassing current technologies as well as methods developed over years of research. Beginning with an introduction to the method, chapters continue with a listing of the materials and equipment, step-by-step protocols, and a Notes section with troubleshooting tips, supplemental details and alternative approaches, as well as a list of references for further information..As part of the practical and convenient .Neuromethods. series, .Extracellular Matrix. serves as an invaluable aid for researchers studying this vi
出版日期Book 2015
关键词Bioengineering; Cell-extracellular matrix interactions; Degeneration and regeneration; Glycosylation; In
版次1
doihttps://doi.org/10.1007/978-1-4939-2083-9
isbn_softcover978-1-4939-4687-7
isbn_ebook978-1-4939-2083-9Series ISSN 0893-2336 Series E-ISSN 1940-6045
issn_series 0893-2336
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

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High-Throughput Quantitative Assay for Analyzing Neurite Outgrowth on a Uniform Substratum: The Cells time and effort. A high-throughput assay provides a quick method to obtain quantitative measures of neurite outgrowth. In this chapter, the experimental setup and imaging protocol are described to efficiently process images of primary neurons or cell lines in order to quantitate parameters.
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Replicating the In Vivo Environment: Organotypic and Submerged Three-Dimensional Culture Methodsrely on modified two-dimensional (2D) substrates. When a cell is attached to a flat surface, the cellular structure is limited by adhesion to the substrate, and the cell is unable to acquire the in vivo morphology and possibly function. In most tissue culture applications, extracellular matrix (ECM)
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Culturing Neurons, Glia, and Progenitor Cells in Three-Dimensional Hydrogelsy relevant cell responses occur in 3D culture environments as opposed to flat stiff culture substrates such as tissue culture plates and coverslips. Hydrogels provide significant advantages towards providing cells a biocompatible, all-encompassing culture environment, but pose challenges towards ada
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Investigating Cell-ECM Interactions and ECM Synthesis in Three-Dimensional Hydrogelsapplied as vehicles for delivering cells for therapeutic applications in vivo. Cells secrete extracellular matrix (ECM), the composition of which depends on the type of cell and cues present; the ECM is assembled and remodeled, forming a dynamic cellular niche. While it is generally recognized that
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