| 书目名称 | Expressed Protein Ligation | | 副标题 | Methods and Protocol | | 编辑 | Miquel Vila-Perelló | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful .Methods in MolecularBiology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troublesho | | 出版日期 | Book 2020 | | 关键词 | protein engineering; split inteins; C-terminal thioesters; hydrazide oxidation; protein splicing | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-0716-0434-2 | | isbn_softcover | 978-1-0716-0436-6 | | isbn_ebook | 978-1-0716-0434-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2020 |
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发表于 2025-3-21 19:42:31
