Titlebook: Expressed Protein Ligation; Methods and Protocol Miquel Vila-Perelló Book 2020 Springer Science+Business Media, LLC, part of Springer Natur

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https://doi.org/10.1007/978-3-7091-9833-9o driven innovations in protein science. Expressed protein ligation (EPL) is a widely used method to generate chemically modified proteins. However, EPL has some limitations, particularly relevant to modify challenging proteins such as antibodies. The method termed streamlined expressed protein liga

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Patricia M. Davies MCSP Dip. Phys. Ed.i of proteins. In this way, protein activity can be preserved while functionalizing the target protein with a wide range of chemical handles. Here, we describe methods for EPL-based modification of proteins produced by yeast, employing an engineered, non–self-cleaving intein known as 202-08. Methods

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https://doi.org/10.1007/978-3-531-92156-3ons form a combinatorial language which defines the functional state of the underlying genome. As each histone type exists in two copies in a nucleosome, the modification patterns can differ between the individual histones, resulting in asymmetry and increasing combinatorial complexity. To systemati

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https://doi.org/10.1007/978-1-0716-0434-2protein engineering; split inteins; C-terminal thioesters; hydrazide oxidation; protein splicing