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Titlebook: Experiments in Molecular Biology; Robert J. Slater Book 1986 Springer Science+Business Media LLC 1986 biology.Colon.DNA.enzyme.enzymes.Esc

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Isolation of Plasmid DNA,e chromosomal DNA, but can contain genes, not only for antibiotic resistance, but also for antibiotic synthesis, toxin production, nitrogen fixation, production of degradative enzymes, and conjugation. The plasmids can be transferred from one cell to another, and therefore act as carriers or “vectors” of the extrachromosomal genes they contain.
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Purification of Plant Ribosomal DNA,ive to main-band and other satellite DNAs so that they can be resolved separately. Cesium chloride-actinomycin D gradients have been used to isolate sea urchin histone genes (.) and plant ribosomal DNA (.), and to separate genes coding for plant 25S and 18S from 5S RNA genes (.) and as a prelude to the cloning of ribosomal DNA (.).
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The Extraction and Affinity Chromatography of Messenger RNA,ing of the control of gene expression, cell division, growth, and development. Furthermore, the recent spectacular advances in genetic engineering depend on a working knowledge of RNA synthesis and its control.
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SDS Polyacrylamide Gel Electrophoresis of Proteins, variation made it difficult to directly compare one sample with another. This problem was overcome by the introduction of the slab gel system (.), in which a large number of samples can be compared on a single run. It is this system that is described in this experiment.
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Book 1986 new laboratory techniques that allow very precise fractionation and analysis of nucleic acids and proteins, as well as the construction of recom­ binant DNA molecules that can then be cloned and expressed in host cells. Progress has been so rapid that there has been a shortfall in the training of a
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