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Titlebook: Experiments in Molecular Biology; Robert J. Slater Book 1986 Springer Science+Business Media LLC 1986 biology.Colon.DNA.enzyme.enzymes.Esc

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发表于 2025-3-21 17:07:15 | 显示全部楼层 |阅读模式
书目名称Experiments in Molecular Biology
编辑Robert J. Slater
视频video
丛书名称Springer Protocols Handbooks
图书封面Titlebook: Experiments in Molecular Biology;  Robert J. Slater Book 1986 Springer Science+Business Media LLC 1986 biology.Colon.DNA.enzyme.enzymes.Esc
描述Research in the field of molecular biology has progressed at a fascinating rate in recent years. Much of this progress results from the development of new laboratory techniques that allow very precise fractionation and analysis of nucleic acids and proteins, as well as the construction of recom­ binant DNA molecules that can then be cloned and expressed in host cells. Progress has been so rapid that there has been a shortfall in the training of appropriately qualified staff. Many existing laboratory workers require retraining, and many educational institutions have had difficulty incor­ porating the new molecular biology techniques into their teaching programs. Although there are several manuals currently available that describe laboratory techniques in molecular biology, they are principally written for the indivi­ dual research worker and are not intended for use in the design of practical classes for students. The aim of this book is to provide just such a series of pro­ tocols forthe teaching of practical molecular biology. The idea arose following the success of several Workshops in Molecular Biology, organized and taught by staff in the Biol­ ogy Department of the Hatfield Po
出版日期Book 1986
关键词biology; Colon; DNA; enzyme; enzymes; Escherichia coli; Filtration; molecular biology; nucleic acid; protein;
版次1
doihttps://doi.org/10.1007/978-1-60327-405-0
isbn_softcover978-0-89603-082-4
isbn_ebook978-1-60327-405-0Series ISSN 1949-2448 Series E-ISSN 1949-2456
issn_series 1949-2448
copyrightSpringer Science+Business Media LLC 1986
The information of publication is updating

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Southern Blotting,The Southern blotting technique was developed by E. Southern in 1975 (.) to facilitate the identification of specific sequences within a complex mixture of DNA fragments. The technique itself has been modified over the years and is now an extremely sensitive and fast analytical technique used in almost every molecular biology laboratory.
发表于 2025-3-22 10:53:26 | 显示全部楼层
Preparative Electrophoresis,Extraction of DNA or RNA fragments from agarose or acrylamide gels is an important tool in genetic engineering. A mixture of fragments can be separated by electrophoresis, and a particular fragment eluted and used for subsequent analysis or cloning.
发表于 2025-3-22 15:25:30 | 显示全部楼层
Vyacheslav Kharchenko,Oleg Illiashenkoodern molecular biology and provide an interesting contrast to the more traditional large-scale biochemical methodologies. The experiment itself will fit into a 3-h practical session and requires no expensive laboratory equipment.
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Microisolation and Microanalysis of Plasmid DNA,odern molecular biology and provide an interesting contrast to the more traditional large-scale biochemical methodologies. The experiment itself will fit into a 3-h practical session and requires no expensive laboratory equipment.
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https://doi.org/10.1007/978-1-60327-405-0biology; Colon; DNA; enzyme; enzymes; Escherichia coli; Filtration; molecular biology; nucleic acid; protein;
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Cleveland Amory and the Fund for Animalss of genetic engineering in theory and would like to supplement their knowledge with practical experience. Many DNA manipulations, however, make use of equipment that is unsuitable for introductory classes with possibly large numbers of students, and require more time than that available in already
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