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Titlebook: Experimental Hematology Today; Siegmund J. Baum (Chairman),G. David Ledney (Head) Book 1977 Springer Science+Business Media New York 1977

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Guangcun He Ph.D.,Bo Du Ph.D.,Rongzhi Chention; and (c) capable of cellular proliferation—including endoreduplication of DNA to produce polyploid cells at various ploidy levels—in addition to cell mitosis. Ultimately, of course, these progenitors must be derived through differentiation or “commitment” of pluripotent hematopoietic stem cells by a process we do not presently understand.
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The Appearance of the Multipotential Hemopoietic Stem Cellrded by hemopoietic cell suspensions was confirmed by many. This soon led to an intensive scientific debate on the mechanism of this protective effect: was it due to a humoral factor produced and provided by the bone marrow—as Lorenz postulated—or to transplantation and subsequent proliferation of hemopoietic cells?
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Physical Separation of the Cycling and Noncycling Compartments of Murine Hemopoietic Stem Cellsre, the cells that form colonies have been termed colony-forming units in the spleen, or CFU-s. The number of CFU-s is then directly proportional to the number of stem cells. The possibility of quantifying the stem cell has led to the initiation of many experiments with the aim of further characterizing it.
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Studies of the Erythroid Inductive Microenvironment in Vitrolonies of apparently undifferentiated cells (4, 8). In the mouse spleen, erythroid colonies outnumber granulocytic colonies 3 to 1 whereas, in the bone marrow. granulocytic colonies outnumber erythroid colonies 2 to 1 (8). The rat spleen supports the growth of erythroid colonies only (18).
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