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Titlebook: Expanded Bed Chromatography; Bo Mattiasson Book 1999 Springer Science+Business Media Dordrecht 1999 Chromatography.DNA.Downstream Processi

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,Konfliktprävention durch Leadership, allows a qualitative and quantitative understanding of the main mechanisms governing the interaction of biomass with fluidised resins. A pulse response technique was used to determine the adsorption of various cell types (yeast, Gram positive and Gram negative bacteria, mammalian cells and yeast ho
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Philosophien und ihr praktischer Nutzen, broad chemical stability. Moreover, combinations of small particle diameters with high densities allow for high fluidization velocities while preserving a rapid mass transfer..This approach has been implemented by combining stable porous mineral oxide of high density (2.2, 4.7, 5.7, 9.4 g/ml) with
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Ziele und Hypothesen der Forschungsstudie STREAMLINE matrix via highly stable ether linkages. Within this development project the chemical and chromatographic stability as well as the breakthrough capacity for human IgG has been studied. The chemical stability was monitored as the carbon leakage from the matrix to the storage solution, pH
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Praktische Anwendung des WVBSW-Modells,ography (IMAC). The 6 × His-tagged protein binds to a chelating resin charged with metal ions such as Ni., Cu. or Zn., and can therefore be separated from proteins which have lower, or no, affinity for the resin. Two recombinant proteins, a malaria transmission-blocking vaccine candidate secreted ex
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Mitregieren im Halbschatten der Verfassung,be the use of expanded bed adsorption for the recovery of a-lactalbumins from defatted milk using the hydrophobic Streamline Phenyl gel. a-Lactalbumins are Ca.- binding proteins. Upon Ca. removal, they undergo a significant conformation change rendering them more hydrophobic. Based on this unique pr
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Grundlegung des Problemhorizontes,tation medium. The pH of the loading and elution buffer were optimised in a packed bed with cell-free culture supernatant. Bound bacteriocin was eluted with 1.0 M NaCl. The highest recovery (30%) was obtained at the lowest pH (3.6). At higher pH values the recovery was lower, namely 12%, 15% and 7%
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Eigenschaften der Generation Y,G. from undiluted and unclarified hybridoma cell culture broth using an ion exchange matrix in expanded bed mode. The tested new sulfopropyl-based ion exchange matrix (Streamline. SP XL, Amersham Pharmacia Biotech) stands out due to its enhanced capacity compared to its precursor (Streamline. SP). D
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