Titlebook: Expanded Bed Chromatography; Bo Mattiasson Book 1999 Springer Science+Business Media Dordrecht 1999 Chromatography.DNA.Downstream Processi

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ious feedstocks comprising bovine albumin in the absence and presence of 20 g dry cell weight biomass litre.. Earlier classification of the simple contactor as a single-stage, well mixed fluidised bed is reviewed. The relative merits of STREAMLINE DEAE and DEAE Spherodex LS as fluidisable, anion exchange adsorbents are discussed.

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Mitregieren im Halbschatten der Verfassung,s are Ca.- binding proteins. Upon Ca. removal, they undergo a significant conformation change rendering them more hydrophobic. Based on this unique property we develop a protocol for fast and efficient purification of α-lactalbumin from milk. The use of this technique results in a reduction of the number of chromatographic purification steps.

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,Verschiedene Einzelausführungen,significantly when elution was carried out at high liquid velocities (300 cm/h) at high sample loads. The eluted peak volume increased with liquid velocity and increased sample load. It was approx. 80% higher in expanded bed mode than in packed bed from an adsorbent completely saturated with protein eluted at 300 cm/h.

HARP

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,Konflikte moderieren und lösen,ost applications. The key to the success of this method seems to be the use of EBA matrices with a relatively low degree of expansion (i.e. a high density) at the linear flow rates employed for elution of bound product.

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