Titlebook: Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation; Narendra Wajapeyee,Romi Gupta Book 2017 Springer Science+B

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书目名称Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation影响因子(影响力)




书目名称Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation影响因子(影响力)学科排名




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书目名称Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation网络公开度学科排名




书目名称Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation被引频次




书目名称Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation被引频次学科排名




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书目名称Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation年度引用学科排名




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书目名称Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation读者反馈学科排名

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High-Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeor template for revealing short-term changes in gene expression, alterations in RNA decay rates, and the kinetics of RNA processing as well as the differentiation thereof. Here, we describe the metabolic labeling and purification of newly transcribed RNA with 4-thiouridine, by which these limitations are overcome.

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Determining if an mRNA is a Substrate of Nonsense-Mediated mRNA Decay in ,,clude detailed methods for growing yeast cells, total RNA isolation, and Northern blotting. Although the chapter focuses on NMD, the methods can be easily adapted to assess the effect of other mRNA decay pathways.

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https://doi.org/10.1007/b110207oliferation, apoptosis, DNA repair, and cell cycle. Therefore, unveiling the mechanisms of eIF3 action in tumorigenesis may help identify attractive targets for cancer therapy. Here, we describe a series of methods used in the study of eIF3 function in regulating protein synthesis, tumorigenesis, and cellular response to therapeutic treatments.

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Alexander M. Polyakov,Fedor K. Popovmutant fungal species. The proteoforms exhibiting differential abundances can be subjected to further targeted studies by other MS or orthogonal (e.g., biochemical) assays. This method can be generally adapted for screening of changes in histone modifications between samples such as wild type vs. mutant or healthy vs. diseased.